I usually wait 24 to 36 hours after washing to condition. Depends on how dry the bag feels. The lunch box I am working on had parts so dry 10 hours later I spot conditioned before going to bed, did the whole bag the next morning and again that night. For the first 3 days, it got spot conditioned in the morning And whole bag at night. It is now down to once a day. In comparison, another bag was washed at 10 a.m. one day and not conditioned until 7 p.m. the next night And was still fairly wet. It only needed 1 conditioning per day.