Ok, so I washed this bag again in the sink and let it soak for quite a while. It has been less than 24 hours and I am beginning to see another stain emerging in a different place in the same general area. The original spot faded a bit but can still be seen. Now I don't know what the heck is going on. Perhaps I am dealing with water spot issues or something. Strange. Wondering if it needs another rinse or if I should just leave it alone.
Could you take some pics of the stains so that we can see them, that might help us give you some ideas.